Fetal Cell Technologies International

FCTI Precursor Stem Cells Source and Types

FCTI uses primary organ culture tissue fragments and/or cell clusters from New Zealand white rabbit origin; in order to insure the cells maintain a diploid set of chromosomes typical of normal somatic cells and the animal sourced organ cultures. FCTI primary tissue culture is sourced from E.U. States certified closed colony of at least 30 generations since 1973.

The cells are extracted with other cells of the same “family” in cell-to-cell contact, including cells of various generations of the same “family”, and then placed into a primary tissue culture to observe activities and perform safety tests, adequate enough to obviate (do away with) any immunogenicity which could cause the need for immunosuppressant drugs. Our cell manufacturing compositions usually consist of the following or any others as and when required :

 

     
1.
LANGERHANS ISLETS
OF PANCREAS
Organ culture, individual cells and cell clusters, islet cells with granules, estimated 80% of total B cells (beta), 10% A (alpha), D and PP cells, and 10% other cells, including stem cells.
     
     
2.
ADRENAL CORTEX Organ culture, 70% of total cells are of the fetal layer, which include stem cells, 20% of fasciculate zone, 10% of glomerularis zone (percentages estimated), round, polyhedral or pyramidal cells, without granules.
     
     
3.
PITUITARY GLAND Organ culture of pars distalis of the gland, some cells are with granules (chromophils), others without (chromophobes). Occasional glial cells ('pituicytes') can be found.
     
     
4.
  THYROID Organ culture containing follicles with colloid, round or cubical cells, less than 5% of total are somewhat larger C    cells with granules. Some parathyroid cells can be found: small chief cells with some granules and rare large oxyphil cells.
     
     
5.
OVARY Organ culture consisting predominately of follicular ('granulose') cells of the primordial follicle.
     
     
6.
TESTIS Organ culture consisting predominately of smaller interstitial Leydig cells, larger Sertoli cells and spermatogenic epithelium.
     
     
7.
PROSTATE Organ culture consisting largely of granular cells originating from seminal vesicles, tubuloalveolar glandular structures, and some smooth muscle fibers.
     
     
8.
STOMACH/INTESTINE Organ culture, primarily cuboidal stem cells, round parietal cells and granular zymogenic cells of stomach, pancreatic stem cells, and of intestinal absorptive cells with brush border, all originating from the mucosa, with a component of lymphoid cells.
     
     
9.
LIVER Organ culture, individual cells, or cell clusters, 90% hepatocytes, remainder consisting of hepatic and hematopoietic stem cells, endothelial cells and some Kupffer cells.
     
     
10.
THYMUS Organ culture consisting predominately of lymphocytes (T- and B-) and epithelial stellate reticular cells.
     
     
11.
KIDNEY Organ culture, primarily of the cortical layer, consisting of round podocytes, larger round to cuboidal cells of convoluted tubules, with and without brush border.
     
     
12.
HEART MYOBLASTS Organ culture, individual cells, or cell clusters, fusiform cells with central nuclei.
     
     
13.
MUSCLE MYOBLAST Organ culture, individual cells, or cell clusters, fusiform cells with peripheral nuclei, with some stem cells with a tendency toward syncytial arrangement.
     
     
14.
SPLEEN Organ culture, network of reticular tissue with lymphoid cell and macrophages, arranged in Billroth cords, with thoroughly washed off blood elements, some flattened sinusoidal endothelial cells.
     
     
15.
LYMPH NODES Organ culture, network of reticular cells with lymphoid cells and macrophages, partially arranged in medullary cords and sinuses.
     
     
16.
LUNG Organ culture dispersed cell clusters of terminal and respiratory bronchiole and of alveolar system, estimated 30% of alveolar cells, 30% of endothelial cells, 30% of interstitial cells, and 10% of macrophages.
     
     
17.
PANCREAS (Even at the fetal stage, 85% of the mass of pancreas is that of an exocrine gland, thus fulfilling that function.) Organ culture, typical serous cells with granules, some disassociated acinar arrangement, and centroacinar stem cells. Also cells of the Langerhans islets.
     
     
18.
MESENCHYME Abundant amorphous ground substance, mostly of hyaluronic acid, pluripotent fibroblasts.
     
     
19.
PLACENTA Organ culture, dispersed cytotrophoblast cells from both fetal and maternal portion, with a few chorionic villi, decidual cells, endothelial cells.
     
     
20.
CARTILAGE Organ culture, prepared from hyaline cartilage, with extracellular matrix with type II collagen, and chondroblasts of attached perichondrium with type I collagen and numerous fibroblasts.
     
     
21.
BONE Organ culture, prepared from cancellous portion of long bones, with multiple osteoblasts from endosteum, also with some hematopoietic stem cells of bone marrow.
     
     
22.
HYPOTHALAMUS/THALAMUS Organ culture, individual cells, and cell clusters, of multipolar smaller neurons of the autonomous nervous system variety, with an abundance of glial cells.
     
     
23.
BASAL GANGLIA Organ culture, individual cells, and cell clusters, of multipolar neurons and abundant glial cells.
     
     
24.
CEREBELLUM Organ culture, individual cells, and cell clusters, consisting of Purkinje cells and very small neurons, some of them stem cells, with an abundance of glial cells.
     
     
25.
CEREBRAL CORTEX Organ culture, individual cells, or cell clusters, consisting of a variety of neurons, including those of pyramidal shape, some of them stem cells, with an abundance of glial cells.
     
     
26.
WHITE MATTER OF BRAIN Organ culture, occasional small neurons, some of them stem cells, more glial cells, fragments of axons.
     
     
27.
BRAIN STEM Organ culture, individual cells, or cell clusters, consisting of a variety of neurons, usually of smaller size and an abundance of glial cells.
     
     
28.
CEREBRUM Organ culture, individual cells, or cell clusters, consisting of a great variety of neurons, some of them stem cells, with an abundance glial cells.
     
     
29.
PINEAL GLAND Organ culture, individual cells, or cell clusters, consisting of melatonin producing pinealocytes and atrocities.